GenBank
Wikipedia 🌐 GenBank
Content
Description
Nucleotide sequences for more than 300,000 organisms with supporting bibliographic and biological annotation.
Data types
captured
Nucleotide sequence
Protein sequence
All
Contact
Primary citation
Release date
1982; 39 years ago
Access
Website
Download URL
ncbi ftp
Web service URL
Tools
BLAST
Miscellaneous
Unclear[1]
The GenBank sequence database is an open access, annotated collection of all publicly available nucleotide sequences and their protein translations. It is produced and maintained by the National Center for Biotechnology Information (NCBI; a part of the National Institutes of Health in the United States) as part of the International Nucleotide Sequence Database Collaboration (INSDC).
GenBank and its collaborators receive sequences produced in laboratories throughout the world from more than 100,000 distinct organisms. The database started in 1982 by Walter Goad and Los Alamos National Laboratory. GenBank has become an important database for research in biological fields and has grown in recent years at an exponential rate by doubling roughly every 18 months.[2][3]
Release 242.0, produced in February 2021, contained over 12 trillion nucleotide bases in more than 2 billion sequences.[4] GenBank is built by direct submissions from individual laboratories, as well as from bulk submissions from large-scale sequencing centers.
Contents
Only original sequences can be submitted to GenBank. Direct submissions are made to GenBank using BankIt, which is a Web-based form, or the stand-alone submission program, Sequin. Upon receipt of a sequence submission, the GenBank staff examines the originality of the data and assigns an accession number to the sequence and performs quality assurance checks. The submissions are then released to the public database, where the entries are retrievable by Entrez or downloadable by FTP. Bulk submissions of Expressed Sequence Tag (EST), Sequence-tagged site (STS), Genome Survey Sequence (GSS), and High-Throughput Genome Sequence (HTGS) data are most often submitted by large-scale sequencing centers. The GenBank direct submissions group also processes complete microbial genome sequences.
Walter Goad of the Theoretical Biology and Biophysics Group at Los Alamos National Laboratory and others established the Los Alamos Sequence Database in 1979, which culminated in 1982 with the creation of the public GenBank.[5] Funding was provided by the National Institutes of Health, the National Science Foundation, the Department of Energy, and the Department of Defense. LANL collaborated on GenBank with the firm Bolt, Beranek, and Newman, and by the end of 1983 more than 2,000 sequences were stored in it.
In the mid 1980s, the Intelligenetics bioinformatics company at Stanford University managed the GenBank project in collaboration with LANL.[6] As one of the earliest bioinformatics community projects on the Internet, the GenBank project started BIOSCI/Bionet news groups for promoting open access communications among bioscientists. During 1989 to 1992, the GenBank project transitioned to the newly created National Center for Biotechnology Information.[7]
Genbank and EMBL: NucleotideSequences 1986/1987 Volumes I to VII.
CDRom of Genbank v100
Growth in GenBank base pairs, 1982 to 2018, on a semi-log scale
The GenBank release notes for release 162.0 (October 2007) state that "from 1982 to the present, the number of bases in GenBank has doubled approximately every 18 months".[4][8] As of 15 June 2019, GenBank release 232.0 has 213,383,758 loci, 329,835,282,370 bases, from 213,383,758 reported sequences.[4]
The GenBank database includes additional data sets that are constructed mechanically from the main sequence data collection, and therefore are excluded from this count.
Organism
Homo sapiens
1.6310774187×1010
Mus musculus
9.974977889×109
Rattus norvegicus
6.521253272×109
Bos taurus
5.386258455×109
Zea mays
5.062731057×109
Sus scrofa
4.88786186×109
Danio rerio
3.120857462×109
Strongylocentrotus purpuratus
1.435236534×109
Macaca mulatta
1.256203101×109
Oryza sativa Japonica Group
1.255686573×109
Nicotiana tabacum
1.197357811×109
Xenopus (Silurana) tropicalis
1.249938611×109
Drosophila melanogaster
1.11996522×109
Pan troglodytes
1.008323292×109
Arabidopsis thaliana
1.144226616×109
Canis lupus familiaris
951,238,343
Vitis vinifera
999,010,073
Gallus gallus
899,631,338
Glycine max
906,638,854
Triticum aestivum
898,689,329
Public databases which may be searched using the National Center for Biotechnology Information Basic Local Alignment Search Tool (NCBI BLAST), lack peer-reviewed sequences of type strains and sequences of non-type strains. On the other hand, while commercial databases potentially contain high-quality filtered sequence data, there are a limited number of reference sequences.
A paper released in the Journal of Clinical Microbiology[10] evaluated the 16S rRNA gene sequencing results analyzed with GenBank in conjunction with other freely available, quality-controlled, web-based public databases, such as the EzTaxon-e[11] and the BIBI [12] databases. The results showed that analyses performed using GenBank combined with EzTaxon-e (kappa = 0.79) were more discriminative than using GenBank (kappa = 0.66) or other databases alone.
^ The download page at UCSC says "NCBI places no restrictions on the use or distribution of the GenBank data. However, some submitters may claim patent, copyright, or other intellectual property rights in all or a portion of the data they have submitted. NCBI is not in a position to assess the validity of such claims, and therefore cannot provide comment or unrestricted permission concerning the use, copying, or distribution of the information contained in GenBank."
^ Benson D; Karsch-Mizrachi, I.; Lipman, D. J.; Ostell, J.; Wheeler, D. L.; et al. (2008). "GenBank". Nucleic Acids Research. 36 (Database): D25–D30. doi:10.1093/nar/gkm929. PMC 2238942. PMID 18073190.
^ Benson D; Karsch-Mizrachi, I.; Lipman, D. J.; Ostell, J.; Sayers, E. W.; et al. (2009). "GenBank". Nucleic Acids Research. 37 (Database): D26–D31. doi:10.1093/nar/gkn723. PMC 2686462. PMID 18940867.
^
^ Hanson, Todd (2000-11-21). "Walter Goad, GenBank founder, dies". Newsbulletin: obituary. Los Alamos National Laboratory.
^ Benton D (1990). "Recent changes in the GenBank On-line Service". Nucleic Acids Research. 18 (6): 1517–1520. doi:10.1093/nar/18.6.1517. PMC 330520. PMID 2326192.
^ Benson, D. A.; Cavanaugh, M.; Clark, K.; Karsch-Mizrachi, I.; Lipman, D. J.; Ostell, J.; Sayers, E. W. (2012). "GenBank". Nucleic Acids Research. 41 (Database issue): D36–D42. doi:10.1093/nar/gks1195. PMC 3531190. PMID 23193287.
^ Benson DA, Karsch-Mizrachi I, Lipman DJ, Ostell J, Sayers EW (January 2011). "GenBank". Nucleic Acids Res. 39(Database issue): D32–37. doi:10.1093/nar/gkq1079. PMC 3013681. PMID 21071399.
^ Kyung Sun Parka, Chang-Seok Kia, Cheol-In Kangb, Yae-Jean Kimc, Doo Ryeon Chungb, Kyong Ran Peckb, Jae-Hoon Songb and Nam Yong Lee (May 2012). "Evaluation of the GenBank, EzTaxon, and BIBI Services for Molecular Identification of Clinical Blood Culture Isolates That Were Unidentifiable or Misidentified by Conventional Methods". J. Clin. Microbiol. 50 (5): 1792–1795. doi:10.1128/JCM.00081-12. PMC 3347139. PMID 22403421.
^ EzTaxon-e Database eztaxon-e.ezbiocloud.net (archive accessed 25 March 2021)
^ leBIBI V5 pbil.univ-lyon1.fr (archive accessed 25 March 2021)
This article incorporates public domain material from the National Center for Biotechnology Information document: "NCBI Handbook".
Sequin — a stand-alone software tool developed by the NCBI for submitting and updating entries to the GenBank sequence database.
EMBOSS — free, open source software for molecular biology
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Databases
Sequence databases: GenBank, European Nucleotide Archive and DNA Data Bank of Japan
Secondary databases: UniProt, database of protein sequences grouping together Swiss-Prot, TrEMBL and Protein Information Resource
Other databases: Protein Data Bank, Ensembl and InterPro
Specialised genomic databases: BOLD, Saccharomyces Genome Database, FlyBase, VectorBase, WormBase, Rat Genome Database, PHI-base, Arabidopsis Information Resource and Zebrafish Information Network
Software
Other
Server: ExPASy
Ontology: Gene Ontology
Institutions
China National GeneBank (CNGB)
Microsoft Research - University of Trento Centre for Computational and Systems Biology (COSBI)
Database Center for Life Science (DBCLS)
DNA Data Bank of Japan (DDBJ)
European Bioinformatics Institute (EMBL-EBI)
J. Craig Venter Institute (JCVI)
Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG)
Philippine Genome Center (PGC)
Organizations
African Society for Bioinformatics and Computational Biology (ASBCB)
Australia Bioinformatics Resource (EMBL-AR)
European Molecular Biology network (EMBnet)
International Nucleotide Sequence Database Collaboration (INSDC)
International Society for Computational Biology (ISCB)
Student Council (ISCB-SC)
Institute of Genomics and Integrative Biology (CSIR-IGIB)
Meetings
File formats
Related topics