ImageStreamX Mk-II

The ImageStreamX (ISx) is a multiparameter imaging cytometer that combines the imaging capability of a wide-field fluorescence microscope and the high throughput, multiparameter ability of a flow cytometer.

The ISX has 12 imaging channels and can acquire up to 9 fluorescent channels from a single sample.

Features

  • 4 lasers (488nm, 561nm, 405nm and 640nm)

  • 12 channels including brightfield and dark field (scatter) channels

  • 9 colour

  • up to 500 events per second

  • 20x, 40x and 60x Olympus objectives

  • 96 well plate reader for automated, unassisted acquisition

  • identification of cells post acquisition to enable effective data analysis

  • Wide array of image analysis tools and protocols

Applications

Resources

Sample Preparation guide (Amnis)

Main points regarding sample preparation:

  • Cells can be live or fixed (acquisition can be quite slow so consider cell health if you are running live cells)

  • Bring single cells suspensions of cells in 100ul of PBS, either in a 1.5mL tube or a 96 well plate

  • Staining protocols are the same as you would perform for flow cytometry

  • Panel design can be a little different so please discuss this with the facility manager prior to commencing any practical work

  • Compensation controls and fluorescence minus one (FMO) controls are advised. Please discuss this with the facility manager ahead of your experiment.

Configuration (Standard)

The configuration is customizable with regards to channels and laser so please feel free to discuss this.

More to come...