ImageStreamX Mk-II
The ImageStreamX (ISx) is a multiparameter imaging cytometer that combines the imaging capability of a wide-field fluorescence microscope and the high throughput, multiparameter ability of a flow cytometer.
The ISX has 12 imaging channels and can acquire up to 9 fluorescent channels from a single sample.
Features
4 lasers (488nm, 561nm, 405nm and 640nm)
12 channels including brightfield and dark field (scatter) channels
9 colour
up to 500 events per second
20x, 40x and 60x Olympus objectives
96 well plate reader for automated, unassisted acquisition
identification of cells post acquisition to enable effective data analysis
Wide array of image analysis tools and protocols
Applications
Resources
Sample Preparation guide (Amnis)
Main points regarding sample preparation:
Cells can be live or fixed (acquisition can be quite slow so consider cell health if you are running live cells)
Bring single cells suspensions of cells in 100ul of PBS, either in a 1.5mL tube or a 96 well plate
Staining protocols are the same as you would perform for flow cytometry
Panel design can be a little different so please discuss this with the facility manager prior to commencing any practical work
Compensation controls and fluorescence minus one (FMO) controls are advised. Please discuss this with the facility manager ahead of your experiment.
Configuration (Standard)
The configuration is customizable with regards to channels and laser so please feel free to discuss this.
More to come...